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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and therefore could lead to tumorigenesis. This protein was discovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial job in promoting mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up claimed but specific nucleotide sequence information and facts isn't yet readily available.
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Blocking peptides are peptides that bind particularly towards the goal antibody and block antibody binding. These peptide normally consists of the epitope identified with the antibody.
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This information outlines further achievable optimization of mobile and immunoassay parameters to make sure the best possible success are...
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This guideline outlines even further achievable optimization of cellular and immunoassay parameters to make sure the best possible results are...
Antibodies sure to the blocking peptide no more bind towards the epitope to the goal protein. By comparing the staining with the blocked antibody compared to the antibody alone, you can see which staining is distinct.
Target Details Cyclin E belongs for the hugely conserved cyclin spouse and children, whose customers are characterized by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation patterns which add into the temporal coordination of every mitotic occasion. Cyclin E varieties a complex with and functions being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates with the G1-S period boundary and is degraded as cells progress by way of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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